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Don elisa test kit
(AG-3061 )

Agriculture Seeds Equipments » Don elisa test kit

Application:

This test kit can be used to detect Deoxynuvalenol in wheat, barley, corn, feed and feed ingredients samples qualitatively and quantitatively.

Technical specifications:

Sensitivity: 5 ppb

Detection limit:

Beer 5 ppb
Corn, peanut, wheat and other food crop, feed 100 ppb
Corn products such as DDGS and corn germ meal, spray corn, Corn gluten meal, finished feed based on the raw materials and concentrate
materials, precision feed 250ppb

Recovery rate:

Beer 90+/-10%
Corn, peanut and other food crop, feed 95+/-15%
Corn products such as DDGS and corn germ meal, spray corn, Corn gluten meal, finished feed based on the raw materials and concentrate
materials, precision feed 95+/-15%
Wheat, grist 80+/-10%

Cross-reaction rate :

Deoxynuvalenol 100%
3-Acetyldeoxynivalenol <1%
Coefficient of variation: <10%

Components:

1) Micro-well strips: 12 strips with 8 removable wells each
2) 6 x concentrated standards 1ml each
0 ppb (zero standard), 10 ppb, 20 ppb, 45 ppb, 135 ppb, 405 ppb
3) Enzyme conjugate 6ml
4) Antibody working solution 6ml
5) Substrate A, 6ml
6) Substrate B 6ml
7) Stop solution 6ml
8) Concentrated washing buffer (10X) 40ml
9) Concentrated sample diluent (5X) 40ml

Materials required but not provided:

1) Equipment: microtiter plate spectrophotometer (450 nm/630nm), vortex (oscillator optional), centrifuge (funnel optional), balance: 0.01g quantity sensitive, gaduated pipettes: 25 ml, polystyrene centrifuge tube: 50m(Triangle bottle with plug optional)
2) Micropipettes: single-channel 10ul ~ 100ul, 100ul ~ 1000ul, eight-channel 300ul
3) Reagents: Methanol, deionized water
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