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Ambala Cantt-133001,Haryana (India)

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Agriculture Seeds Equipments

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Total Aflatoxin ELISA Test Kit

AG-3060

Application:

This test kit can be used to detect total aflatoxins in maize, corn, feed, edible oil and other samples qualitatively and quantitatively.

Technical specifications

Detection limit: 0.1 ppb

Recovery rate:

Peanuts : 95+/-15%
Animal feed : 85+/-15%
Edible oil : 95+/-15%
Corn : 90+/-15%

Cross-reaction rate:

Aflatoxin B2 : 100%
Aflatoxin B1 : 97%
Aflatoxin G1 : 98%
AflatoxinG2 : 101%
AflatoxinM1 : 30%

Components :

1) Micro-well strips: 12 strips with 8 removable wells each
2) 6xstandards,0 ppb(zero standard), 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb,8.1ppb-1ml each
3) 1 x High concentration standard,100ppb 1ml
4) Enzyme conjugate 10 ml
5) Antibody working solution 6 ml
6) Substrate A 10ml
7) Substrate B 10m
8) Stop solution 6ml
9) Concentrated washing buffer 40ml

Materials required but not provided:

1) Equipment: microtiter plate spectrophotometer (450 nm/630nm), shaker, vortex Shaker, centrifuge, water bath, balance: 0.01g quantity sensitive, gaduated pipettes: 10ml, rubber suction bulb, polystyrene centrifuge tube: 10ml,50ml, filter funnel, Rotary evaporator/nitrogen evaporator, Glass Centrifuge Tube:10ml, filter
2) Micropipettors: single-channel 20-200 uL, 100-1000 uL, and multi-channel 250 uL
3) Reagents: methanol, distilled or deionized water, petroleum ether /n-hexane

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